Synonyms / Designations: Kisspeptin-10, Metastin (10-10), KiSS-1–derived decapeptide, KISS1 receptor ligand fragment
CAS Number: 374675-21-5
Molecular Formula: C63H83N17O14
Molecular Weight: ~1302.4 g/mol
Peptide Classification: Synthetic decapeptide; KISS1-derived neuropeptide fragment
Purity: ≥ 99 % (HPLC, typical)
Appearance: White to off-white lyophilised powder
Pack Size: 10 mg (total)
Storage: Desiccated, protected from light, stored at –20 °C
Solubility: Soluble in sterile water and buffered aqueous solutions
Description & Mechanism
Kisspeptin-10 is a synthetic decapeptide corresponding to the C-terminal region of the endogenous KiSS-1 gene product. It represents the minimal bioactive fragment commonly used in research settings to study kisspeptin-mediated signalling independent of longer precursor peptides.
In biochemical and cellular research systems, Kisspeptin-10 interacts with the KISS1 receptor (also known as GPR54), a G-protein-coupled receptor expressed in hypothalamic and peripheral tissues. Experimental studies investigate receptor binding, downstream signalling cascades, and neuroendocrine pathway activation under controlled conditions. Observed molecular responses are dependent on experimental design, concentration, and model system, and are used to examine receptor-mediated signalling rather than defined physiological or clinical outcomes.
Applications in Research
- As a molecular probe for studying KISS1 receptor binding and activation
- Investigation of hypothalamic–pituitary–gonadal (HPG) axis signalling pathways
- In vitro studies examining neuropeptide-mediated receptor signalling
- Use as a reference compound in neuroendocrine and reproductive signalling research
Handling, Reconstitution & Stability
- Weigh under dry conditions; peptide is hygroscopic
- Reconstitute in sterile water or appropriate buffered aqueous solution depending on assay requirements
- Avoid vigorous agitation during dissolution
- Filter sterilize if required (e.g. 0.22 µm) immediately prior to use
- Aliquot and store reconstituted solutions at –20 °C (or lower) to minimise degradation
- Avoid repeated freeze–thaw cycles
Specifications Summary
| Parameter |
Typical Value / Range |
| Purity (HPLC) |
≥ 99 % |
| Appearance |
White to off-white lyophilised powder |
| Molecular Weight |
~1,302.4 g/mol |
| Peptide Type |
Synthetic KISS1-derived decapeptide |
| Solubility |
Water, buffered aqueous solutions |
| Storage |
–20 °C, desiccated, dark |
| Pack Size |
10 mg |
Precautions & Notes
- Experimental behaviour of Kisspeptin-10 is influenced by receptor expression, concentration, and exposure duration
- Neuropeptide signalling responses may vary significantly across model systems
- Buffer composition, pH, and ionic strength may affect peptide stability and assay performance
- Appropriate controls are recommended to distinguish receptor-specific from non-specific effects
- Intended strictly for laboratory research use; not for human or veterinary application
References
Kotani M. et al. The metastasis suppressor gene KiSS-1 encodes kisspeptins, the natural ligands of the orphan G protein-coupled receptor GPR54. Journal of Biological Chemistry, 2001.
https://pubmed.ncbi.nlm.nih.gov/11451936/
Seminara S.B. et al. The GPR54 gene as a regulator of puberty. New England Journal of Medicine, 2003.
https://pubmed.ncbi.nlm.nih.gov/12571259/
Dhillo W.S. et al. Kisspeptin-54 stimulates the hypothalamic-pituitary-gonadal axis in human males. Journal of Clinical Endocrinology & Metabolism, 2005.
https://pubmed.ncbi.nlm.nih.gov/15998775/
Pinilla L. et al. Kisspeptins and reproduction: physiological roles and regulatory mechanisms. Physiological Reviews, 2012.
https://pubmed.ncbi.nlm.nih.gov/22396373/
Keywords: Kisspeptin-10, Metastin, KISS1 Peptide, GPR54 Ligand, Synthetic Neuropeptide
