Synonyms / Designations: Cardiogen; AEDR; cardiac (heart) peptide bioregulator
Amino Acid Sequence: Ala-Glu-Asp-Arg (AEDR)
CAS Number: N/A
Molecular Formula: C18H31N7O9
Molecular Weight: 489.49 g/mol
Purity: ≥ 99 % (HPLC)
Appearance: White to off-white lyophilised powder
Pack Size: 20 mg (total)
Storage: Desiccated, protected from light, stored at –20 °C or below
Solubility: Soluble in water and buffered aqueous solutions
Description & Mechanism
Cardiogen is a short synthetic tetrapeptide employed as a molecular probe for investigating tissue-specific transcriptional regulation in cardiac-derived cell and tissue systems. It is used experimentally to study modulation of gene-expression programmes in cardiomyocyte and cardiac-fibroblast models, including transcripts associated with contractile proteins, calcium handling and oxidative-stress defence.
Consistent with the Khavinson short-peptide model, Cardiogen is studied as an agent that may translocate intracellularly and engage DNA/chromatin to influence promoter activity. As a low-molecular-weight linear peptide it is used in experimental designs focused on transcriptional regulation and cellular stress signalling; observed effects depend on concentration, model system and exposure conditions.
Applications in Research
- In-vitro gene-expression studies in cardiomyocyte and cardiac-fibroblast cultures
- Cellular models of oxidative stress and cardiomyocyte apoptosis / protein synthesis
- Preclinical models of cardiac aging and ischaemia-related gene-expression changes
- Probe for short-peptide / chromatin interaction studies
Specifications Summary
| Parameter |
Typical Value / Range |
| Purity (HPLC) |
≥ 99 % |
| Appearance |
White to off-white lyophilised powder |
| Molecular Weight |
489.49 g/mol |
| Peptide Type |
Synthetic linear tetrapeptide bioregulator (Khavinson cytogen family) |
| Solubility |
Water, buffered aqueous solutions |
| Storage |
–20 °C, desiccated, dark |
| Pack Size |
20 mg (total) |
Handling, Reconstitution & Stability
- Weigh under dry conditions; peptide is hygroscopic
- Reconstitute in sterile water or appropriate buffered aqueous solution depending on assay requirements
- Avoid vigorous agitation during dissolution
- Filter sterilize if required (e.g. 0.22 µm) prior to use
- Aliquot and store reconstituted solutions at –20 °C or below to minimise degradation
- Avoid repeated freeze–thaw cycles
Precautions & Notes
- Experimental behaviour is dependent on concentration, model system and exposure conditions
- Appropriate experimental controls are recommended
- Prolonged exposure to light, elevated temperature or aqueous environments may reduce stability
- Intended strictly for laboratory research use; not for human or veterinary application
References
Khavinson VKh, Linkova NS, Tarnovskaya SI, et al. Short cell-penetrating peptides: a model of interactions with gene promoter sites. Bull Exp Biol Med.
https://pubmed.ncbi.nlm.nih.gov/23484211/
Short Peptides Regulate Gene Expression. Bull Exp Biol Med. 2016.
https://link.springer.com/article/10.1007/s10517-016-3596-7
Khavinson VKh, Popovich IG, et al. Peptide Regulation of Gene Expression: A Systematic Review. Molecules. 2021;26(22):7053.
https://www.mdpi.com/1420-3049/26/22/7053
PubChem Compound Summary, CID 11583989 (Ala-Glu-Asp-Arg).
https://pubchem.ncbi.nlm.nih.gov/compound/11583989
Keywords: Cardiogen, AEDR, cardiac peptide bioregulator, synthetic tetrapeptide
